Review



yeast total cellular protein extracts  (Kamada)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Kamada yeast total cellular protein extracts
    Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote yeast mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by <t>immunoblotting.</t> Equivalent percentage amounts (2%) of total cellular protein extracts were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909
    Yeast Total Cellular Protein Extracts, supplied by Kamada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+total+cellular+protein+extracts/yeast+total+cellular+protein+extracts/pmc05869690-177-5-17
    Average 90 stars, based on 1 article reviews
    yeast total cellular protein extracts - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Yeast Ste23p shares functional similarities with mammalian insulin-degrading enzymes"

    Article Title: Yeast Ste23p shares functional similarities with mammalian insulin-degrading enzymes

    Journal: Yeast (Chichester, England)

    doi: 10.1002/yea.1709

    Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote yeast mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by immunoblotting. Equivalent percentage amounts (2%) of total cellular protein extracts were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909
    Figure Legend Snippet: Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote yeast mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by immunoblotting. Equivalent percentage amounts (2%) of total cellular protein extracts were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909

    Techniques Used: Sequencing, Expressing, Functional Assay, Plasmid Preparation, Selection, Western Blot, SDS Page, Control, Transformation Assay

    Ste23p is expressed in both haploid and diploid yeast. Plasmid-based expression of Ste23p (A) and Axl1p (B) was examined in MATa (IH1783), MATα (IH1784) and diploid (IH1788) cell types, using pWS482 and pWS371, respectively. The steady-state levels of the indicated HA-tagged protein (top panel) and yeast actin (bottom panel) were detected by immunoblotting as described in Figure 6, except that 60% of the total cellular extract preparation was evaluated in the instance of Axl1p samples, due to its low abundance
    Figure Legend Snippet: Ste23p is expressed in both haploid and diploid yeast. Plasmid-based expression of Ste23p (A) and Axl1p (B) was examined in MATa (IH1783), MATα (IH1784) and diploid (IH1788) cell types, using pWS482 and pWS371, respectively. The steady-state levels of the indicated HA-tagged protein (top panel) and yeast actin (bottom panel) were detected by immunoblotting as described in Figure 6, except that 60% of the total cellular extract preparation was evaluated in the instance of Axl1p samples, due to its low abundance

    Techniques Used: Plasmid Preparation, Expressing, Western Blot

    Related Articles

    Western Blot:

    Article Title: Yeast Ste23p shares functional similarities with mammalian insulin-degrading enzymes
    Article Snippet: .. Preparation of cell extracts and immunoblotting Yeast total cellular protein extracts were prepared as previously described ( Fujimura-Kamada et al. , 1997 ). ..



    Similar Products

    yeast total cellular protein extracts  (Kamada)
    90
    Kamada yeast total cellular protein extracts
    Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote yeast mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by <t>immunoblotting.</t> Equivalent percentage amounts (2%) of total cellular protein extracts were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909
    Yeast Total Cellular Protein Extracts, supplied by Kamada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+total+cellular+protein+extracts/yeast+total+cellular+protein+extracts/pmc05869690-177-5-17
    Average 90 stars, based on 1 article reviews
    yeast total cellular protein extracts - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote yeast mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by immunoblotting. Equivalent percentage amounts (2%) of total cellular protein extracts were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909

    Journal: Yeast (Chichester, England)

    Article Title: Yeast Ste23p shares functional similarities with mammalian insulin-degrading enzymes

    doi: 10.1002/yea.1709

    Figure Lengend Snippet: Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote yeast mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by immunoblotting. Equivalent percentage amounts (2%) of total cellular protein extracts were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909

    Article Snippet: Preparation of cell extracts and immunoblotting Yeast total cellular protein extracts were prepared as previously described ( Fujimura-Kamada et al. , 1997 ).

    Techniques: Sequencing, Expressing, Functional Assay, Plasmid Preparation, Selection, Western Blot, SDS Page, Control, Transformation Assay

    Ste23p is expressed in both haploid and diploid yeast. Plasmid-based expression of Ste23p (A) and Axl1p (B) was examined in MATa (IH1783), MATα (IH1784) and diploid (IH1788) cell types, using pWS482 and pWS371, respectively. The steady-state levels of the indicated HA-tagged protein (top panel) and yeast actin (bottom panel) were detected by immunoblotting as described in Figure 6, except that 60% of the total cellular extract preparation was evaluated in the instance of Axl1p samples, due to its low abundance

    Journal: Yeast (Chichester, England)

    Article Title: Yeast Ste23p shares functional similarities with mammalian insulin-degrading enzymes

    doi: 10.1002/yea.1709

    Figure Lengend Snippet: Ste23p is expressed in both haploid and diploid yeast. Plasmid-based expression of Ste23p (A) and Axl1p (B) was examined in MATa (IH1783), MATα (IH1784) and diploid (IH1788) cell types, using pWS482 and pWS371, respectively. The steady-state levels of the indicated HA-tagged protein (top panel) and yeast actin (bottom panel) were detected by immunoblotting as described in Figure 6, except that 60% of the total cellular extract preparation was evaluated in the instance of Axl1p samples, due to its low abundance

    Article Snippet: Preparation of cell extracts and immunoblotting Yeast total cellular protein extracts were prepared as previously described ( Fujimura-Kamada et al. , 1997 ).

    Techniques: Plasmid Preparation, Expressing, Western Blot